The assay and reaction kinetics of leucine aminopeptidase from swine kidney.
نویسندگان
چکیده
Danielli, J. F. (1952) Symp. Soc. exp. Biol. 6, 1. Deul, D. H. & McIlwain, H. (1961). Biochem. J. 80, 19P. Dingle, J. T. & Lucy, J. A. (1963). Biochem. J. 86, 15P. Florey, W. H. & Grant, L. H. (1961). J. Path. Bact. 82, 13. Hirsch, J. G. (1962). J. exp. Med. 116, 827. Hokin, L. E. & Hokin, M. R. (1958). J. biol. Chem. 233, 805. Hokin, M. R., Benfey, B. G. & Hokin, L. E. (1958). J. biol. Chem. 233, 814. Kennedy, E. P. (1962). Harvey Led. 57, 157. Kimizuka, H. & Koketsu, K. (1962). Nature, Lond., 196, 995. Lowry, 0. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). J. biol. Chem. 193, 265. Millonig, G. (1961). J. biophy8. biochem. Cytol. 11, 736. Naora, H., Naora, H., Mirsky, A. E. & Allfrey, V. G. (1961). J. gen. Phy8iol. 44, 713. Pease, D. 0. (1960). Hi8tological Techniques for Electron Micro8copy, p. 45. London: Academic Press (Inc.) Ltd. Woodin, A. M. (1961). Biochem. J. 80, 562. Woodin, A. M. (1962). Biochem. J. 82, 9. Woodin, A. M. (1963). Symp. biochem. Soc. 22, 126. Woodin, A. M., French, J. E. & Marchesi, V. T. (1963). Biochem. J. 87, 567. Woodin, A. M. & Wieneke, A. A. (1963a). Biochem. J. 87, 480. Woodin, A. M. & Wieneke, A. A. (1963b). Biochem. J. 87, 487.
منابع مشابه
Leucine aminopeptidase. VII. Action on long chain polypeptides and proteins.
The availability of highly purified leucine aminopeptidase (LAP)’ from swine kidney (3) has made it possible to study the action of this enzyme on protein and polypeptide substrates. By analogy with its action on amino acid amides and small peptides, the aminopeptidase should possess no endopeptidase activity and should hydrolyze only those peptide bonds which are adjacent to a free a-amino gro...
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As long ago as 1905, Warburg (1) employed the amino acid esterase activity of tissues to prepare the optical isomers of amino acids. Since that time, other than for the studies of esterase activity of the proteases, there have been only scattered reports of the amino acid esterase activity of tissue extracts. In fact, Smith and coworkers (24) have emphasized that their preparations of “leucine ...
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OBJECTIVES Changes in the activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L in the liver and kidney of male and female of mice, injected with 0.4 IU/kg b.w. insulin for 4 and 8 days. METHODS The homogenates of the liver and kidney were taken for examination. The activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L has been determine...
متن کاملStopped-flow cryoenzymology: detection of catalytic intermediates not observable at ambient temperatures.
Stopped-flow cryoenzymology has been used to study the reaction of porcine kidney cytosol leucine aminopeptidase [alpha-aminoacyl-peptide hydrolase(cytosol), EC 3.4.11.1] with L-leucylglycyldansyl hydrazide in 50% (vol/vol) methanol buffer over the -40 to 23 degrees C temperature range. Resonance energy transfer between tryptophan residues of the enzyme E and the dansyl group of the substrate S...
متن کاملA continuous spectrophotometric method for the determination of leucine aminopeptidase.
A new method to assay the enzyme, leucine aminopeptidase (LAP; L-leucyl-peptide hydrolase.-3.4.1.1), is described. The technic is an extension of the conventional glutamic-pyruvic transaminase (GPT) reaction. Serum LAP is allowed to react with the substrate L-leucyl-L-alanine to quantitatively generate alanine. This alanine, in the presence of excess -ketoglutaric acid and GPT, rapidly forms py...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 90 3 شماره
صفحات -
تاریخ انتشار 1964